Part:BBa_K133083:Design

CMV-SS-HA-UreB-gyrEC-2xlinker-(TMTIR)TLR9-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2918
  Illegal BamHI site found at 1790
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1012
  Illegal AgeI site found at 2190
  Illegal AgeI site found at 2590
  Illegal AgeI site found at 2728
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3604
  Illegal SapI.rc site found at 898
  

Design Notes

The part is cloned into BBa_J52017, a vector with eukaryotic terminator.